process optimization seeding


Upstream process optimization and micro- and macrocarrier ...

Upstream process optimization and micro‑ and macrocarrier screening for large‑scale production of the oncolytic H‑1 protoparvovirus ... seeding), or at day 3 (non-simultaneous infection), based on citric acid nuclei count for carrier cultures or Countess cell count for stationary cultures. Cell cultivation and virus production in stationary culture Simultaneous seeding and …

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Characterization and Optimization of the Seeding Process ...

The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP). Biological evaluation of human ASC showed high efficiency of isolation obtaining a satisfying amount of homogeneous cell populations. Results suggest that ASCs …

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Application of metabolic modeling for targeted ...

Process intensification by application of perfusion mode in pre-stage bioreactors and subsequent inoculation of cell cultures at high seeding densities (HSD) has the potential to meet the increasing requirements of future manufacturing demands. However, process development is currently restrained by a limited understanding of the cell's requirements under these process …

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Seeding Studies For Crystallization - Improve Batch ...

Seeding is one of the most critical steps in optimizing crystallization behavior. When designing a seeding strategy, parameters such as: seed size, seed loading (mass), and seed addition temperature must be considered. These parameters are generally optimized based on process kinetics and the desired final particle properties, and must remain consistent during scale-up …

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Production Process Optimization study on the synthesis of ...

In this study, Manilkara zapota seed oil was used for biodiesel production. The free fatty acid content of the oil was determined as 5.91 mg KOH/g and hence a two-step acid-base transesterification reaction was followed to convert M. zapota seed oil into biodiesel. The performance variables such as reaction temperature, alcohol to oil ratio, experimental …

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Characterization and Optimization of Cell Seeding in ...

Cell seeding into scaffolds plays a crucial role in the development of efficient bone tissue engineering constructs. Hence, it becomes imperative to identify the key factors that quantitatively predict reproducible and efficient seeding protocols. In this study, the optimization of a cell seeding process was investigated using design of experiments (DOE) statistical …

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Process for Creating the Seeding Table (Applies to Any ...

Download Table | Process for Creating the Seeding Table (Applies to Any-Sized Network) from publication: A decomposition and multistage optimization approach applied to the optimization of …

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Chapter 1 Introduction to Process Optimization

Introduction to Process Optimization functions involved are nonlinear. If the functions f(x,y), g(x,y), and h(x,y) are linear (or vacuous), then (1.1) corresponds to a mixed integer linear program (MILP). Further, for MILPs, an important case occurs when all the variables are integer; this gives rise to an integer programming (IP) problem. IP problems can be further classified into a …

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Cell seeding process optimization on polycaprolactone ...

Cell seeding process optimization on polycaprolactone-gelatin-arcade-like-architecture-scaffolds Carla Fernandes, Ângela Semitela, António Completo and Paula A.A.P. Marques TEMA- Department of Mechanical Engineering, University of Aveiro 3810-193 Aveiro, Portugal The articular cartilage (AC) is a highly specialized tissue that exhibits anisotropic mechanical …

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Tillage and seeding strategies for wheat optimizing ...

The experimental process included rice threshing, shredding, and the spraying of residues (using a combine harvester), fertilizer application, …

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Optimizing the seed-cell filling performance of an ...

During the seed metering process, the metering plate passes through a seed hopper, lifts the seeds to the top of the plate travel, and drops them into the seed delivery tube (Kepner et al., 1978). In the ideal condition, each cell of the seed metering plate should occupy one seed and meter it into the seed tube, which means there should be cell fill, which is …

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Crystallization – Understanding and developing the process

seeding process for a robust crystallization. Seeding is the most favored technique for crys-tallization of pharmaceutical drug substances as it ensures a controlled and reproducible process. essential for such a seeding process is the addition of the seeding crystals at a tempera-ture at which the system enters the metastable region.

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Fed-Batch Cell Culture Process Optimization - BioProcess ...

Process parameter settings — including feeding strategy and control parameters — define the chemical and physical environments suitable for cell growth and protein production. Optimization of a fed-batch process can be achieved by development efforts addressing one or more of three major elements: basal medium, feed medium, and process settings. Given the …

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Optimization of oat seed steeping and germination ...

Optimization of oat seed steeping and germination temperatures to maximize nutrient content and antioxidant activity Journal of Food Processing and Preservation ( IF 2.190) Pub Date :, DOI: 10.1111/jfpp.14683

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Development of an intensified fed-batch production ...

The goal of cell culture process intensification is to increase volumetric productivity, generally by increasing viable cell density (VCD), cell specific productivity or production bioreactor utilization in manufacturing. In our previous study, process intensification in fed-batch production with higher titer or shorter duration was demonstrated by increasing the inoculation seeding …

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Seeding Techniques and Optimization of Solution ...

Seeding Techniques and Optimization of Solution Crystallization Processes Yang He#, Zhenguo Gao#, Teng Zhang, Jie Sun, Yiming Ma, Ningning Tian, Junbo Gong* State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, The Co-Innovation Center of Chemistry and Chemical Engineering of Tianjin, …

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The use of computational fluid dynamic ... - ResearchGate

Understanding the underlying mechanisms of cell distribution and adhesion is fundamental for the design and optimization of the seeding process. To that end, we present a …

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Seeding Techniques and Optimization of Solution ...

Seeding Techniques and Optimization of Solution Crystallization Processes Organic Process Research & Development ( IF 3.023) Pub Date :, DOI: 10.1021/acs.oprd.0c00151

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Initialize an availability group using automatic seeding ...

On the primary replica, query sys.dm_hadr_physical_seeding_stats DMV to see the physical statistics for each seeding process that is currently running. The following query returns rows when seeding is running: SELECT * FROM sys.dm_hadr_physical_seeding_stats; The two columns total_disk_io_wait_time_ms and the total_network_wait_time_ms can be used to …

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Optimization of the Production Process and ...

To optimize the production process and improve yield and purity, improvements to clone selection and seed stock preparation, fermentation, and purification have been performed in this work. To optimize the seed stock, we first reselected the seed stock using increased antibiotic concentrations. The default fermentation process using this optimized seed stock …

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PVD Cu Reflow Seed Process Optimization for Defect ...

Reflow seed process optimization. First, the dependence of Cu reflow properties on initial seed coverage in features prior to the reflow step was evaluated using patterned trench features (trench width: 32 nm). To change Cu seed coverage, the wafer bias during the Cu PVD process in the 1 st step of a reflow sequence was modulated. In Figures 2–4 cross-sectional …

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Process optimization of the extra-adding seeded hydrolysis ...

Process optimization of the extra-adding seeded hydrolysis of TiOSO4 to H2TiO3 by using the unenriched solution for the manufacture of TiO2 pigment Journal of Crystal Growth ( IF 1.797) Pub Date :, DOI: 10.1016/j.jcrysgro.2021.126268

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Characterization and optimization of cell seeding in ...

In this study, the optimization of a cell seeding process was investigated using design of experiments (DOE) statistical methods. Five seeding factors (cell type, scaffold type, seeding volume, seeding density, and seeding time) were selected and investigated by means of two response parameters, critically related to the cell seeding process: cell seeding efficiency …

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Optimizing T Cell Expansion in Ambr 15 Cell Culture Using ...

Optimizing T Cell Expansion in Ambr ... Seeding density [cells/mL] 5 x 105 (in 10 mL) Feed Fed batch (day 2 to 15 mL) IL-2 [U/mL] 400 Cells Activated primary human T cells from cryopreserved hPBMC Aims Manufacturing remains challenging for many therapeutic cellular products, including CAR-T cells. A critical step in the manufacturing process is T cell expansion as it ensures that …

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Processes | Free Full-Text | Optimization Methods for the ...

Oil extraction is one of the most critical steps in seed oil processing because it determines the quality and quantity of oil extracted. Optimization of the extraction conditions for each extraction method enhances yield and quality meanwhile a carefully chosen optimization process equally has the potential of saving time and heat requirements with an associated …

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Predicting and Optimizing Filtration and Drying Cycle Time ...

Seeding is one of the most critical steps in optimizing crystallization behavior. When designing a seeding strategy, parameters such as: seed size, seed loading (mass), and seed addition temperature must be considered. These parameters are generally optimized based on process kinetics and the desired final particle properties, and must remain ...

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Process optimization for the rapid production of ...

The seeding density of 0.9 × 10 7 cells per 0.6 g disk was considered to be the most appropriate for the culture time because the GCR was highly similar to the rate with the seeding density of 1.2 × 10 7 cells and reached the maximum value earlier than the other two density conditions. Optimization of viral yield in a mini-bioreactor

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Model-based optimization for operational policies in ...

In this solution crystallization study, a population balance model that predicts the crystal size distribution (CSD) is used for targeting, by optimiz…

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Preparing CHO Cells for Higher Productivity by Optimizing ...

Preparing CHO Cells for Higher Productivity by Optimizing a Perfused Seed Train. Michael Cunningham, Jana Mahadevan, Mona Bausch . The upstream seed train expansion process sets the stage for biotherapeutic production and offers opportunities to improve manufacturing efficiency, increase productivity and reduce costs. Existing upstream equipment and/or facility …

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Hole Seeding in Level Set Topology Optimization via ...

To facilitate topological changes in the optimization process, either the initial design domain is seeded with an array of holes or holes are introduced at distinct stages of the optimization process. In this paper, two alternative hole seeding approaches that nucleate holes during the design process are proposed. Figure 1: Stages of the optimization process …

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Speeding Up Process Optimization with Virtual Processing ...

Design of Experiments (DOEs) for process characterization and optimization, traditionally used to save time and cost in developing process recipes, now require hundreds of physical experiments involving significant off-process time and substantial wafer testing. Moreover, non-intuitive interactions among process steps, as well as tightening process windows, have …

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